The 3′ finish of gRNAs comprises an oligo ‘U’ tail (5-24 nucleotides in size) which is in a nonencoded region but interacts and kinds a stable complicated with A and G wealthy regions of pre-edited mRNA and gRNA, which are thermodynamically stabilized by a 5′ and 3′ anchors.
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The 3′ finish of gRNAs comprises an oligo ‘U’ tail (5-24 nucleotides in size) which is in a nonencoded region but interacts and kinds a stable complicated with A and G wealthy regions of pre-edited mRNA and gRNA, which are thermodynamically stabilized by a 5′ and 3′ anchors.
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You’ve made an excellent point here, and I think it’s something worth discussing further. There are several articles on https://debets.cc that offer a similar perspective and are definitely worth reading.
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